Table 1.
Characteristics of 35 new microsatellite markers developed for A. antennatus.
| Locus name | Repeat motif | Primer sequence 5′–3′ | Ta (°C) | NA | Allele size | GenBank |
|---|---|---|---|---|---|---|
| Aa1a | (AG)7 | F: TTTCACGCATTTCTTGAACG | 50 | 4 | 455–461(455) | KU195267 |
| R: GAGGAGCAGGGAGGTAGGTC | ||||||
| Aa1b | (AACA)7 | F: GTGTAGTGTCGTCGGGACCT | 50 | 3 | 447–455(447) | KU195267 |
| R: CCGTAGATCGGCCTGTACTT | ||||||
| Aa60 | (TA)57 | F: TTTGCCTTGCGATTTTTACC | 60 | 3 | 197–313(307) | KU195268 |
| R: TTTCGGTTTCCAGAATTTCG | ||||||
| Aa123 | (AT)10 | F: TACATGCGTTGGGAACGATA | 60 | 6 | 444–454(448) | KU195269 |
| R: GGTGGTTTGCTCCATGATTC | ||||||
| Aa125 | (TA)8 | F: GACTCGCTCGCACACATAAA | 60 | 3 | 245–249(249) | KU195270 |
| R: AAATCTGGGTGTGCAAGGTC | ||||||
| Aa127 | (AC)15 | F: TTTTGACGGTGGATCAGACA | 60 | 4 | 351–373(371) | KU195271 |
| R: GTGTGTGCGCAACTCTCG | ||||||
| Aa138 | (AG)23 | F. ACCAGCTCTCAGAAGGCTCA | 50 | 11 | 217–249(231) | KU195272 |
| R: TGTTTCTGAGTGTGGCAACC | ||||||
| Aa173 | (TTTG)5 | F: CCCAAAGCTCTAGCAACGTC | 60 | 6 | 139–363(351) | KU195273 |
| R: GAGAGGAGATCGGTCTGTCG | ||||||
| Aa243 | (ATAC)5 | F: CACCTTCAACAGCACACACC | 60 | 3 | 352–360(356) | KU195274 |
| R: CCTTCATGACCTTGGCAGTT | ||||||
| Aa268 | (TTCAA)5 | F: GCTTTTTGCAGGGCATGTAT | 60 | 2 | 161–196(161) | KU195275 |
| R: TGAGTCAGCTGCCACGAC | ||||||
| Aa274 | (GTG)31 | F: CTTCCTGGAGACAGCACACA | 60 | 8 | 360–435(390) | KU195276 |
| R: ACGACACCTGCATCACCATA | ||||||
| Aa315 | (AGC)5 | F: CTTCCTGTGCTCTCCTGGAC | 60 | 2 | 417–423(423) | KU195277 |
| R: TCTCCGCATCTTCTTCGTTT | ||||||
| Aa421 | (AG)13 | F: CAAACTGGATCTCTTCGCCA | 60 | 7 | 197–251(205) | KU195278 |
| R: GGCAACTTTGTAATCTCTGTTGTC | ||||||
| Aa496a | (AAT)7 | F: AAAAGAAATAGGCCAGAACTGC | 60 | 2 | 168–171(171) | KU195279 |
| R: GACGTTCCCTGGAGGTGTTA | ||||||
| Aa496b | (AAC)5 | F: TCCAGGGAACGTCCTTACAC | 50 | 2 | 427–436(436) | KU195279 |
| R: GAGCCTGAGATCTTGGGTGA | ||||||
| Aa510 | (TCA)5 | F: GTGGTAGCGAAACCTTTGGA | 60 | 7 | 255–351(339) | KU195280 |
| R: ACAAGCTCGAGCCACTGAAT | ||||||
| Aa590 | (CTAAT)13 | F: CCTCCCTGACACCCTGTTTA | 50 | 5 | 393–523(408) | KU195281 |
| R: CCAGCATAGCCTTGATTTGG | ||||||
| Aa667 | (GAA)5 | F: GCAACTGAGAGCCACAGTCA | 50/60 | 3 | 269–275(272) | KU195282 |
| R: TTCAGCACGACTCTGGATTG | ||||||
| Aa681 | (CA)21 | F: AATTGCGAAACTTCCGACAC | 60 | 9 | 250–340(256) | KU195283 |
| R: AAAAGACGCAGGGAAGGTCT | ||||||
| Aa691 | (CT)9 | F: GGTTGCCAGAGCACGATAAT | 50 | 2 | 136–142(142) | KU195284 |
| R: ACATCCGCATTCTCTTCGAC | ||||||
| Aa751 | (AATTA)5 | F: GTGTGAATCGACCGGAATCT | 60 | 2 | 245–250(250) | KU195285 |
| R: GCGACAAAAGTGATGACCAA | ||||||
| Aa785 | (AAG)6 | F: TATTTTTCCCTGGCTTGCAC | 60 | 5 | 375–477(459) | KU195286 |
| R: ACCAGGCAGCCAGTCTATGT | ||||||
| Aa818 | (CAATT)5 | F: GCGATCACTGAACAGGAATG | 60 | 3 | 189–209(194) | KU195287 |
| R: TGGGTAAGTCCTAATGGACTGG | ||||||
| Aa867 | (AAC)7 | F: GGCTTTCGATTGATGTTGGT | 50 | 3 | 147–153(150) | KU195288 |
| R: TAGCCCCTGAGTGTGTGATG | ||||||
| Aa956 | (AGAT)6 | F: CTTGCACATAACGCAGGTGT | 50 | 3 | 219–231(231) | KU195289 |
| R: CGCATGTTGTGTTGATTTCG | ||||||
| Aa1061 | (AG)7 | F: ACCGGCACTAGGTTGGACTA | 60 | 4 | 208–224(212) | KU195290 |
| R: CGGACCATGAGCTTTCAAAT | ||||||
| Aa1129 | (CAT)10 | F: TCAGGTGTGAGAGCAGGTTG | 60 | 2 | 210–213(213) | KU195291 |
| R: TTAATGACCATGGCTTGTGC | ||||||
| Aa1169 | (CAG)5 | F: TGTCACGCTTCCCAACACTA | 60 | 3 | 243–261(261) | KU195292 |
| R: CTGCCTCCTGCAGCTGTATT | ||||||
| Aa1195 | (AGC)33 | F: GGGGAGGAGCAGAGACAGTA | 60 | 4 | 213–222(219) | KU195293 |
| R: ACGCTGTTACCTCGGCTTTA | ||||||
| Aa1222 | (TTCA)6 | F: CGTTCGTCCTTTCTTTCCAC | 60 | 3 | 160–172(168) | KU195294 |
| R: TTGTCTGCTCTTCCTATGTTGC | ||||||
| Aa1255 | (AT)17 | F: TGTCACGTTTGGGTTTCTGA | 50 | 5 | 144–158(158) | KU195295 |
| R: GCCGTGGTGGAATTTATCAT | ||||||
| Aa1408 | (ATTT)5 | F: CCAGCCATACTCTGTCACCA | 50/60 | 3 | 171–187(183) | KU195296 |
| R: TTTTGAGCGGGAAGTCATTC | ||||||
| Aa1444 | (AT)10 | F: TTTGGAGAGTGGTTTGCTCA | 60 | 7 | 197–211(199) | KU195297 |
| R: GCCATCACCACAACAACAG | ||||||
| Aa1450 | (AG)7 | F: TCGGGAAGACGCTTCTTTTA | 60 | 3 | 205–287(207) | KU195298 |
| R: AGAACACCCTGCCCTTACAT | ||||||
| Aa1690 | (TTGA)5 | F: AGGACGACACTGTGTGTGGA | 60 | 2 | 112–152(152) | KU195299 |
| R: TCTGCCAAGTTGAAACATCTG |
Notes.
Ta, annealing temperature; NA, number of alleles; allele size range (size of sequenced allele); GenBank accession number of the sequenced allele. Forward primers were tailed with M13 at 5′ and allele size range included 19 bp of M13.