Figure 4. PoT protocol processed for the removal of bacterial endotoxins by 1-Octanol and solvent removal by dialysis and speed vacuum.

(A) The PoT protocol processed for endotoxin removal by the dialysis and speed vacuum methods generated a 7.2 mL (3.8 × 10¹⁰ PFU·ml⁻¹) and 4.2 mL (4.7 × 10¹⁰ PFU·ml⁻¹) phage banks, respectively. (B) Endotoxin concentrations of dialysis (14 EU·ml⁻¹) and speed vacuum (27 EU·ml⁻¹) processed PoT phage banks. Quantification of 1-Octanol treated concentrate was not possible due to residual solvent disabling the LAL quantification test, dotted line represent the desired 20 EU·ml⁻¹ cut off.