Table 8.
Berberine synergistically inhibits MexXY-mediated gentamicin resistance in P. aeruginosa mutants.
| Strain | XY | AAC | MIC (μg/ml) for GEN in the presence of: | MIC (μg/ml) for BB in the presence of: | FIC | Mode of Interaction | ||
|---|---|---|---|---|---|---|---|---|
| − | +BB | − | +GEN | |||||
| PAGUg1927 | + | + | 1024 | 256 | >512 | 128 | <0.5 | Synergy |
| PAGUg1931 | − | + | 8 | 16 | >512 | >512 | >2.0 | Indifferent |
| PAGUg1929 | + | − | 8 | 2 | >512 | 128 | <0.5 | Synergy |
| PAGUg1933 | − | − | 0.25 | 0.25 | >512 | >512 | >1.0 | Indifferent |
GEN, gentamicin; BB, berberine; XY, mexXY; AAC, aacC1*.
*aacC1 is a gentamycin acetyl transferase-encoding gene derived from pPS858 (Hoang et al., 1998).