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. 2016 Aug 1;9:4773–4783. doi: 10.2147/OTT.S103940

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© 2016 Chen et al. This work is published and licensed by Dove Medical Press Limited

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Armillarikin-induced apoptotic cell death of human HCC cells.

Notes: (A and B) Huh7 and (B, right panel) HepG2 cells were treated with DMSO or armillarikin for 3 days, and the percentage of apoptotic cells (hypodiploid cells) with DNA ladders was detected using flow cytometry. **P<0.01 and ***P<0.001 (Student’s t-test) compared with DMSO control. (C) Armillarikin-induced micronuclei morphology. After Huh7 cells had been treated with armillarikin for 3 days, they were fixed and stained with Hoechst. Fluorescent and cell images were captured; arrow heads indicate micronuclei 400×.

Abbreviations: DMSO, dimethyl sulfoxide; HCC, hepatocellular carcinoma.

Armillarikin-induced apoptotic cell death of human HCC cells.

Notes: (A and B) Huh7 and (B, right panel) HepG2 cells were treated with DMSO or armillarikin for 3 days, and the percentage of apoptotic cells (hypodiploid cells) with DNA ladders was detected using flow cytometry. **P<0.01 and ***P<0.001 (Student’s t-test) compared with DMSO control. (C) Armillarikin-induced micronuclei morphology. After Huh7 cells had been treated with armillarikin for 3 days, they were fixed and stained with Hoechst. Fluorescent and cell images were captured; arrow heads indicate micronuclei 400×.

Abbreviations: DMSO, dimethyl sulfoxide; HCC, hepatocellular carcinoma.