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. 2016 Aug 1;9:4773–4783. doi: 10.2147/OTT.S103940

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© 2016 Chen et al. This work is published and licensed by Dove Medical Press Limited

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Z-VAD-fmk inhibited cell death in armillarikin-treated human HCC cells.

Notes: (A) HA22T and (B) Huh7 HCC cells were treated with DMSO or 6.25–50 µM armillarikin with or without of 20 µM Z-VAD-fmk (pan-caspase inhibitor) for 48 or 72 hours. The relative number of viable cells was detected using MTS assay. *P<0.05, **P<0.01, and ***P<0.001 (Student’s t-test) compared with Z-VAD-fmk treatment.

Abbreviations: DMSO, dimethyl sulfoxide; HCC, hepatocellular carcinoma; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium.