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. 2016 Aug 1;9:4763–4771. doi: 10.2147/OTT.S102101

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© 2016 Zhang et al. This work is published and licensed by Dove Medical Press Limited

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Flow cytometric analysis of apoptosis of MHCC-H cells.

Notes: MHCC-H cells treated with CON, DOC, DLLMs, and DLLMs + UTMD for 48 h were harvested (n=6/group). (A) Apoptosis assay by flow cytometric analysis. (B) Quantification of apoptosis. (C) Western blot assay of cleaved caspase-3 and quantification from three independent experiments. Mean ± standard deviation is shown. *P<0.05 and **P<0.01.

Abbreviations: CON, control; DOC, docetaxel; DLLMs, docetaxel-loaded lipid microbubbles; UTMD, ultrasound-targeted microbubble destruction; h, hours; FITC, fluorescein isothiocyanate.