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Representative dot plots of coated-platelet measurement in a normal sample on flow cytometer. Platelets were gated (P1) based on FSC-SSC parameters, and then these events were further analyzed in P2 gate where only CD41-positive cells were counted (92%). Finally, coated-platelets were separated from the rest of platelets according to their increased fibrinogen binding detected by biotinylated- fibrinogen and streptavidin-PE (38%; P3).
