Skip to main content
. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Nat Cell Biol. 2015 Aug 24;17(9):1145–1157. doi: 10.1038/ncb3227

Figure 4.

Figure 4

The basolateral junctional complex promotes anchorage-independent growth and expression of transformation-related markers. (a) Caco2 control, PLEKHA7 knockdown (PLEKHA7 shRNA) and PLEKHA7– p120 double knockdown (PLEKHA7 shRNA, p120 shRNA) cells were grown on soft agar and quantified for colony formation (mean ± s.d. from n = 3 independent experiments; *P = 0.0008 Student’s two-tailed t-test; see Supplementary Fig. 4c for representative scan). Source data are provided in Supplementary Table 2. (b) Control, PLEKHA7 knockdown (PLEKHA7 shRNA) and PLEKHA7–p120 double knockdown (PLEKHA7 shRNA, p120 shRNA) Caco2 cells were analysed by western blot for the markers shown. Actin is the loading control (see also Supplementary Fig. 4d). (c) Control, PLEKHA7 knockdown (PLEKHA7 shRNA) and PLEKHA7/E-cadherin (PLEKHA7 shRNA, Ecad shRNA) double knockdown Caco2 cells were analysed by western blot for the markers shown. Actin is the loading control. (d) Western blot of Caco2 control or PLEKHA7 knockdown (PLEKHA7 shRNA) cells after treatment with vehicle control (dimethylsulphoxide, DMSO) or 10 μM Src inhibitor PP2; actin is the loading control (see also Supplementary Fig. 4e). (e) Western blot of p120 knockdown Caco2 cells (p120 shRNA) after ectopic expression of either vector control (vector), murine full-length (isoform 1A) wild-type p120 (mp120-1A) or murine full-length non-phosphorylatable p120 (mp120-8F) for the markers shown. Actin is the loading control. (f) Western blot of single and double PLEKHA7 (PLEKHA7 shRNA) and cadherin 11 (Cad11 shRNA) knockdown Caco2 cells for the markers shown. Actin is the loading control. (g) IF of control (NT) or PLEKHA7 knockdown (PLEKHA7 shRNA) cells for PLEKHA7 and cadherin 11 (Cad11). Scale bar: 20 μm. See Supplementary Fig. 8 for unprocessed blot scans of b–f.