Table 1. Transportome hits which downregulate oxygen consumption >10%.
| Gene/ Gene ID | Inhibition OCR CA, % | Cell Index, % | Affymetrix expression | Name and function |
|---|---|---|---|---|
| KCNN43783 | 22 | 93 | 1.00 | Intermediate conductance calcium-activated potassium channel protein 4, KCa3.1 |
| SLC26A21836 | 21 | 94 | 1.00 | Sulfate transporter. |
| NEDD4L23327 | 20 | 95 | 1.00 | E3 ubiquitin-protein ligase NEDD4-like |
| SLC19A210560 | 20 | 84 | 1.00 | Thiamine transporter 1 |
| PSEN15663 | 17 | 72 | 1.00 | Presenilin-1; L-glutamate transport; gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein) |
| SLC2A14144195 | 17 | 94 | 1.00 | GLUT14, GLUT3 duplication. Solute carrier family 2, facilitated glucose transporter member 14 |
| KCNT2343450 | 16 | 96 | 0.43 | Potassium channel subfamily T member 2 |
| SLC25A3281034 | 16 | 129 | 1.00 | Mitochondrial folate transporter/carrier; Transports folate across the inner membranes of mitochondria. |
| SLC25A123560 | 15 | 102 | 1.00 | Tricarboxylate transport protein, mitochondrial. Involved in citrate-H+/malate exchange. Important for the bioenergetics of hepatic cells as it provides a carbon source for fatty acid and sterol biosyntheses, and NAD+ for the glycolytic pathway. |
| SCO29997 | 13 | 106 | 1.00 | Acts as a copper chaperone, transporting copper to the Cu(A) site on the cytochrome c oxidase subunit II (COX2), complex IV assembly |
| SLC12A26558 | 12 | 93 | 1.00 | Basolateral Na-K-Cl symporter |
| SLC1A46509 | 11 | 97 | 0.93 | ASCT1 protein, Neutral amino acid transporter A; Transporter for alanine, serine, cysteine, and threonine. Exhibits sodium dependence. |
| BSG682 | 10 | 82 | 1.00 | Basigin; Major regulator of SLC16A1, SLC16A3 and SLC16A8 activity |
OCR: oxygen consumption rate; CA: cell number adjusted; Cell index: Changes in cell number compared to control transfected cells.