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. 2016 Aug 5;11(8):e0160418. doi: 10.1371/journal.pone.0160418

Fig 1. Construction of M&R LE protein marker for Western blotting.

Fig 1

We fused mouse and rabbit linear eptitopes (M&R LE) to generate a 15 kDa leader peptide which can be recognized by anti-mouse or anti-rabbit IgG secondary antibodies. The M&R LE molecular weight ladder was constructed by fusing a leader peptide with the E. coli outer membrane protein intimin domain 2 (D2), maltose-binding protein (MBP) or E. coli transcription factor (Nus) polypeptides. The M&R LE protein marker can be directly visualized on Western blotting by staining with anti-mouse or anti-rabbit IgG secondary antibodies. SP, signal peptide; M2R2, M&R epitope; RE, restriction enzyme sites; MAS, MW adjusting sequence; H, 6X His tag.