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. 2016 Aug 5;11(8):e0160418. doi: 10.1371/journal.pone.0160418

Fig 3. Expression and recognition of M&R LE leader peptides by mouse and rabbit secondary antibodies.

Fig 3

The LE candidates (M1, M2, M3, R1, R2 and R3) and M&R LE leader peptide (M2R2) were separated by a 12.5% SDS-PAGE and transfered onto NC membrane. The expression of polypeptides was detected by staining with (A) anti-His tag antibody and standard Western Blotting. The recognition of LE candidates and M&R LE leader peptides on immunoblots by (B) HRP-conjugated anti-mouse or (C) HRP-conjugated anti-rabbit IgG Fc secondary antibodies. The chemiluminescence was captured by a UVP BioImaging system. The relative molecular weight (kDa) of commercial prestained markers is indicated on the left.