Table 2. Commercial secondary antibodies tested for recognition of the M&R LE protein marker in Western Blots.
| Secondary antibody | Auto-detected activity | Company (Catalog no.) |
|---|---|---|
| Goat anti-mouse IgG Fc-HRP | Yes | Jackson lab (115-035-008) |
| Goat anti-rabbit IgG Fc-HRP | Yes | Jackson lab (111-035-008) |
| Goat anti-mouse IgG (H+L)-HRP | Yes | Invitrogen (626520) |
| Goat anti-mouse IgG (H+L)-HRP | Yes | Thermo (31430) |
| Goat anti-rabbit IgG (H+L)-HRP | Yes | Invitrogen (656120) |
| Goat anti-rabbit IgG (H+L)-HRP | Yes | Thermo (31460) |
| Goat anti-mouse IgG + IgM (H+L)-HRP | Yes | Jackson lab (115-035-044) |
| Goat anti-rabbit IgG + IgM (H+L)-HRP | Yes | Bio-Rad (401005) |
| Goat anti-mouse IgM-HRP | n.d. | Jackson lab (115-035-020) |
| Goat anti-rabbit IgM-HRP | n.d. | Abcam (Ab97195) |
| Goat anti-mouse IgG F(ab’)2-HRP | n.d. | Jackson lab (115-036-006) |
| Goat anti-rabbit IgG F(ab’)2-HRP | n.d. | SantaCruz (sc-3837) |
n.d., not detected