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. 2016 Aug 5;11(8):e0160246. doi: 10.1371/journal.pone.0160246

Fig 2. AtPRORP1-mediated cleavage of pre-tRNASerSu1 (pSu1).

Fig 2

Representative gel showing AtPRORP1-mediated cleavage of pre-tRNASerSu1 (pSu1) substrates with and without the 3' CCA. Lanes 1 to 8 represent negative controls (absence of AtPRORP1), and M (size marker, lane 9) indicates cleavage of pATSerUG by Eco RPR. Note that this cleavage generates a 5' cleavage fragment (5' CL Frags) one nucleotide longer compared to that generated during cleavage of pSu1. Lanes 10 and 14 pSu1(-1A), lanes 11 and 15 pSu1(-1C), lanes 12 and 16 pSu1(-1G), and lanes 13 and 17 pSu1(-1U). The final concentration of AtPRORP1 was 0.37 μM and the reactions were performed at 37°C for 30 s in the presence of 10 mM Mg2+ (see Materials and Methods).