Fig 5. AtPRORP1-mediated cleavage of pATSer variants.

Representative gel showing AtPRORP1-mediated cleavage of 3' CCA-motif-containing pATSer variants. Lanes 1 to 6 and 14 to 17 are negative controls (loaded in the same order as the reactions with AtPRORP1 in lanes 8 to 13 and 19 to 22, respectively); and lanes 7 and 18 (size marker) refers to cleavage of pATSerUG by Eco RPR. The final concentration of AtPRORP1 was 6.6 μM and the reactions were performed at 37°C for 60 min in the presence of 10 mM Mg²⁺. The position of each 5' cleavage fragment (5' CL Frags) generated after cleavage is indicated. The two lower panels represent overexposure to better highlight the 5'-cleavage products in the upper panels. (Note: Fig A in S1 File shows cleavage of pATSer derivatives without the 3' CCA-motif.)