Table 3. Comparison of substrate recognition attributes of bacterial RNase P (RNP) and PRORPs.

Pre-tRNA location	Role in catalysis	Bacterial RNase P	PRORP	References
5'-leader	Substrate recognition	From N-1 to N-7	Only N-1 and N-2	[17, 18, 51]
N-1 identity	Cleavage fidelity and efficiency	Yes		This study and [7, 15, 17]
2'-OH in N-1	Cleavage efficiency	Yes		This study and [31, 32]
G+1 as positive and G-1 as negative determinants	Cleavage-site selection	Yes	No	This study and [14, 15, 29]
N-1:N73 base pairing	Cleavage fidelity	Yes		This study and [14, 15, 17, 18, 29]
D-stem/loop	Rate of cleavage	Moderate	Significant	This study and [4, 11, 17, 18, 21]
T-stem/loop	Rate of cleavage	Significant		This study and [4, 11, 17, 21]
3'-CCA motif	Substrate recognition and cleavage fidelity	Yes	No	This study and [9, 13, 17, 37]