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. 2016 Aug 5;11(8):e0160233. doi: 10.1371/journal.pone.0160233

Fig 8. LDH expression is up-regulated in cut(ue)-Gal4 > lov RNAi larvae.

Fig 8

Late wandering third instar larvae were collected for RNA extraction. Semi-Q RT-PCR was used for transcript analysis. LDH and actin PCR products for each RNA sample were run in parallel in separate gel lanes. 1 and 2 indicate RT-PCR products from two independent RNA preparations. Four RNA preparations total gave comparable results. For quantitation of LDH upregulation in cut(ue)-Gal4 > lov RNAi larvae, band intensities for the LDH and actin PCR products were quantitated with NIH image J and used to calculate LDH/actin (L/a) ratios for control and experimental (cut(ue)-Gal4 > lov RNAi) samples. The fold increase in LDH expression in the experimental samples was then calculated as the average value for L/a experimental/L/a control.