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. 2016 Aug 5;12(8):e1005811. doi: 10.1371/journal.ppat.1005811

Fig 1. PP2C38 associates dynamically with the EFR-BIK1 and FLS2-BIK1 complexes.

Fig 1

(A-B) Co-immunoprecipitation of PP2C38 and EFR (A) or FLS2 (B) transiently expressed in N. benthamiana leaves treated (+) or not (-) with 100 nM elf18 (A) or flg22 (B) for 20 min. (C) Co-immunoprecipitation of PP2C38 and FLS2 in stable transgenic Arabidopsis seedlings (T3) expressing PP2C38-GFP. Seedlings were treated (+) or not (-) with 1 μM flg22 for 20 min. Native FLS2 protein visualized in immunoblot using α-FLS2 antibody. The PM marker GFP-LTI6b [71] was used as a negative control. (D) Co-immunoprecipitation of PP2C38 and BIK1 or BAK1 transiently expressed in N. benthamiana leaves treated (+) or not (-) with 100 nM flg22 for 20 min. (E) Co-immunoprecipitation of PP2C38 and BIK1 transiently expressed in Arabidopsis Col-0 protoplasts. Protoplasts were treated (+) or not (-) with 1 μM elf18 for 30 min. All experiments were performed at least three times with similar results.