Fig 4. Effect of lichen enriched media and incubation time on the growth of P. furfuracea bacterial strains.

The 100% stacked column chart of growth of 139 P. furfuracea bacterial strains on the minimal medium AB supplemented, or not, with lichen extract (L) and/or with carbon sources (glucose, G; mannitol, M; or glucose and mannitol, GM) after 24 h (a) and 96 h (b) of incubation at 25°C under dark conditions. Bacterial growth on each assayed medium was compared with control medium KB, which was arbitrarily set at 100% growth. Results were grouped in three categories (no growth, growth on the inoculated spot and abundant growth on the inoculated spot). Data are from one experiment performed by duplicate. Significant (p<0.001) differences between two culture media are indicated by asterisks above stacked columns. Significant (p<0.001) differences were also found between 24 h (a) and 96 h (b) of incubation.