Figure four. Increased inflammatory cytokine production by NOD.ABD CD8 T cells.

(a) CD4 and CD8 T cells were purified from NOD.ABD and control B10.H2^g7 splenocytes as described in Materials and Methods, and 1×10⁵ cells cultured with or without CD3 and CD28 antibodies at 37°C for 3 days. Concentrations of IFN-γ and TNF-α were determined by CBA (see Materials and Methods). Data represent the average values of triplicate samples ± SD and were consistent in two repetitions. (*, p<0.05, NOD.ABD vs. B10.H2^g7 ). (b,c) Liver lymphoid cells were isolated and stained intracellularly as described in the Materials and Methods. The frequency of IFN-γ positive cells in CD4⁺TCRβ⁺ and CD8⁺TCRβ⁺ cell populations were analyzed by flow cytometry. One representative example of IFN-γ in CD4⁺ and CD8⁺ T cells is shown in (b). The frequencies of IFN-γ in CD4⁺ and CD8⁺ T cells are shown in (c). Data are shown as the means ± SD. Each group included 4 mice (*, NOD.ABD vs. B10.H2^g7, p<0.05).