Figure 1. Development of p75^NTR in the rat cerebellum.

(a) Low magnification images of sagittal sections through the entire cerebellum from postnatal day (P) 2 through P21 showing the abundant immunolabeling for p75^NTR in the EGL, which decreases by P21. Size bar indicates either 200 µm or 400 µm, as indicated. (b) High magnification images of lobe 6 showing p75^NTR labeling in the outer EGL. Arrows indicate the inner EGL where the neurons lack p75^NTR. Size bar indicates 10 µm and is the same for all the images in B. (c) Western blot of cerebellum lysates from the indicated ages. Tissue was lysed with RIPA buffer containing protease inhibitors and 20 µg of total protein was separated on a 10% gel and probed for p75^NTR. The gel was re-probed for actin as a loading control and is representative of 3 independent experiments.

DOI: http://dx.doi.org/10.7554/eLife.16654.003

Figure 1—figure supplement 1. No differences in TUNEL labeling in the EGL between Ngfr-/- and WT mice.

Sections from WT or Ngfr-/- mouse P7 cerebellum were processed for TUNEL labeling. Few labeled cells were detected and no difference between genotypes was observed.

DOI: http://dx.doi.org/10.7554/eLife.16654.004