Figure 4. Mature neurotrophins have no effect on proliferation of cultured P7 rat GCPs in the absence or presence of Shh.

GCPs from P7 rat were cultured with BrdU for 48 hr in the absence or presence of Shh with or without the different mature neurotrophins. BrdU labeling was analyzed by in-cell Western on the LiCor Odyssey. (a) GCPs with or without 20 ng/ml or 100 ng/ml of NGF. (b) GCPs with or without 20 ng/ml or 100 ng/ml of BDNF. (c) GCPs with or without 20 ng/ml or 100 ng/ml of NT3. (d) GCPs with or without 20 ng/ml or 100 ng/ml of NT4. Data are expressed as mean +/- SEM from three independent experiments. Asterisk indicates that Shh-treated cells increased BrdU incorporation compared to controls by ANOVA with Tukey’s posthoc analysis, p values are indicated below each graph.

DOI: http://dx.doi.org/10.7554/eLife.16654.008