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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2016 May 27;15(8):1809–1822. doi: 10.1158/1535-7163.MCT-15-0688

Figure 5. MLN8237 reduces metastatic colonization and improves overall survival.

Figure 5

A Experimental design to test impact of MLN8237 on metastatic colonization. B Representative H&E images of lung tissue (48h), white line outline tumor cell/s, scale bar-10μm. C Quantification of single cell/clusters metastases as in (B) 24–72 hours, two mice/time point, three independent experiments, one-way ANOVA: vehicle/MLN8237. D Quantification of total number of metastases as in (A–B), one-way ANOVA, vehicle/MLN8237. E Experimental design to test impact of MLN8237 on overall survival in orthotopic models. Representative BLI images of mice before and after the surgery. Average radiance/ROI, fold of change; all animals in this study died from pulmonary metastatic outgrowth. No difference in BLI/or pathology was documented at the point of euthanasia. F Kaplan-Meier survival curves, mice as in (E) treated in adjuvant setting, 8 mice/group, n=2. G Percent of animals as in (E) with recurrent tumors, identified during necropsy; paired t-test. H Experimental design to test the impact of MLN8237 on overall survival in tail vein model of systemic disease spread. I Representative BLI images of mice 1–3 weeks post injection; Quantification of average radiance, fold of change (graph, right); one-way ANOVA. Imaging at later time points was not possible due to respiratory distress-caused death in vehicle group. J Kaplan-Maier survival curves, mice as in (H) treated with vehicle or MLN8237, 12 mice/group, two independent experiments; representative H&E images of metastases at the end point. K Experimental design to test the impact of MLN8237 in combination with chloroquine on metastatic colonization. L Quantification of average radiance/lungs, fold of change; treatment as indicated for 2 weeks, n=16, one-way ANOVA. M Quantification of metastases at the end point/mm2, n=6 lungs/group, one-way ANOVA. N Experimental design to test the impact of MLN8237 on lung metastases in orthotopic model using PDXs. O Quantification of total number of metastases as in (K) immediately after 2 weeks of treatment, multiple t-tests, Holm-Sidak method, vehicle/MLN8237; representative H&E images of metastases at the end point, black arrows indicate lung metastasis.