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Western blot analysis of total cortical membrane pSer552-NHE-3 protein expression following 1 day of control (
), systemic ANG II (sANG II;
), sANG II + systemic C-21 (sC-21;
), and sANG II + intrarenal (IR) C-21 (
) treatments. All blots are normalized to GAPDH. Data represent mean ± 1 SE. **P<0.01from control. ++P<0.001 from sANG II.
