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. Author manuscript; available in PMC: 2017 Aug 4.
Published in final edited form as: Mol Cell. 2016 Jul 28;63(3):470–484. doi: 10.1016/j.molcel.2016.06.035

Figure 3. ZMYND8 knockdown increases the invasive abilities of prostate cancer cells in vitro and in vivo.

Figure 3

(A) Western blot and quantitative RT-PCR analysis of ZMYND8 levels following the treatment of DU145 cells with shZMYND8s (shZMYND8-95 and shZMYND8-97). (B–D) Effects of ZMYND8 knockdown on the proliferation (B), migration (C) and invasion (D) of DU145 cells. Following the cell migration and invasion assays, cells were stained with crystal violet and counted in at least five fields. (E–G) The effect of ZMYND8 knockdown on the in vivo metastatic abilities of DU145 cells in an intravenous mouse xenograft model. DU145 cells with stably expressing firefly luciferase were infected with lentiviruses containing scramble shRNA (shScramble) or shZMYND8-97. The representative bioluminescent images of mice (shScramble, n=6; shZMYND8, n=6) 8-10 weeks after tail vein injection are shown (E), and their quantified bioluminescent signals were individually plotted (F). Representative images of hematoxylin and eosin–stained lung tissues (G). Data are presented as the mean ± SEM (error bars). See also Figure S1.