5-μm-thick paraffin slides from reconstituted skin at day 21 were processed for immunofluorescent staining of following markers: K14 for basal epidermis (control: A; mutant: E), K10 for suprabasal epidermis (control: B; mutant F), K15 for hair follicle stem cells and secondary hair germ (control: C; mutant G), AE13 for hair keratins (control: D; mutant: H), Gata3 for inner root sheath (control: I; mutant: M), Sox 9 for outer root sheath (control: J; mutant: N), Versican for anagen DP (control: K; mutant: O) and Sox2 (control: L; mutant: P). Sections were nuclear counterstained with DAPI (blue). Images shown are representative of at least three replicates. Scale bars: 200 μm.