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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Feb 12;1859(9):1170–1182. doi: 10.1016/j.bbagrm.2016.02.008

Figure 4. The HDAC3 Deacetylase Enzyme Affects PXR-SUMOylation and Co-localizes with PXR in Hepatocytes.

Figure 4

Figure 4

(A) Hepa1-6 cells were transfected with the indicated plasmid expression vectors. SUMOylated proteins were captured using cobalt beads and the extent of RFP-PXR modification was analyzed using western blotting with an anti-PXR antibody. (B) Primary cultures of mouse hepatocytes were transfected with RFP-PXR and HDAC3-GFP. Twenty-four hr post-transfection, hepatocytes were treated with rifampicin (10 μM), trichostatin A (0.5 μM), or both for an additional 24 hr. Fluorescent cells were imaged as described under Materials and Methods. To facilitate the visualization of the nucleus Hoechst 33342 (3 μM) was added to the live cells thirty min prior to imaging.