Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Lancet Infect Dis. 2016 Feb 6;16(5):556–564. doi: 10.1016/S1473-3099(15)00464-8

Figure 2. Representative oppA1 PCR melting temperature peaks of Borrelia genospecies.

Figure 2

Representative melting temperature peaks in °C for B afzelii (A peak; acceptable range 51·7–56·7°C), novel B burgdorferi sensu lato genospecies (B peaks; 60·38–61·24°C), and B burgdorferi sensu stricto (C peaks; 61·7–66·7°C). Y-axis represents the negative derivative of the ratio of the FRET signal (LC-Red640 flouresence) and background fluorescein fluorescence.