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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Lancet Infect Dis. 2016 Feb 6;16(5):556–564. doi: 10.1016/S1473-3099(15)00464-8

Figure 4. Phylogenetic analyses.

Figure 4

(A) Phylogenetic analysis of a 149 base pair fragment of the oppA1 gene amplified from patient specimens (MN14-1539, MN14-1420, WI133, ND132, and ND121) and tick specimens (CP12150 and EC10N1) compared with seven different species of the B burgdorferi sensu lato complex. There is no homologous sequence in relapsing fever borreliae. Bootstrap support values greater than 50% are shown. The scale bar corresponds to 0·01 substitutions per nucleotide position. Accession numbers are indicated for available Borrelia species oppA1 sequences retrieved from GenBank. The B americana BAA-1877 oppA1 gene sequence was generated in this study. GenBank does not allow deposition of sequences shorter than 200 bp; oppA1 sequences generated in this study are available by request. (B) Phylogenetic analysis of eight concatenated housekeeping genes: uvrA, rplB, recG, pyrG, pepX, clpX, clpA, and nifS, amplified from patient isolates (MN14-1539, MN14-1420) compared with 18 different B burgdorferi sensu lato genospecies and three relapsing fever species. Bootstrap support values greater than 50% are shown. The scale bar corresponds to 0·1 substitutions per nucleotide position. The source of other Borrelia species gene sequences is shown in the supplemental methods. Sequence nomenclature (eg, MN14-1539, WI133) represents the state from which the diagnostic specimens were submitted for testing and does not necessarily show the patient’s state of residence.