Skip to main content
. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Feb 23;1859(9):1155–1169. doi: 10.1016/j.bbagrm.2016.01.006

Fig. 2.

Fig. 2

p300 and SIRT1 modulate PXR's transcriptional activity. 293T cells were co-transfected with pSG5-PXR, CYP3A4-Luc (A, C, E, F) or MDR1-Luc reporter (B, D), and pCMV-β-galactosidase control plasmids. 24 h after transfection, cells were treated with DMSO, Rif, or SR12813 as indicated for an additional 24 h. (A, B) Cells were co-transfected with pCDNA3.1. empty vector control, pCDNA3.1-p300, or pCDNA3.1-p300 ΔHAT mutant as well. (C, D) Cells were co-transfected with pCDNA3.1. empty vector control, pECE-SIRT1, or pECE-SIRT1 H363Y. (E) Transfected cells were treated with increasing concentrations of TSA (E), or NAM (F) at the same time with DMSO or RIF as indicated. Cells were then lysed and luciferase and β-galactosidase activities were measured. Luciferase values were normalized to β-galactosidase activities. The data represents the mean and SEM of at least 3 independent experiments performed in triplicate. ****p < 0.05, n.s., not significant 2-way ANOVA. Rif = rifampicin, TSA = Trichostatin A, NAM = nicotinamide.