Table 2.
Glycopeptides identified from proteinase K digest of flagellin following ETD MS/MS (without FAIMS)
| Peptide sequence | Glycans | Site | Charge state | m/z meas | m/z calc | Δ ppm | CV | #1 | #2 |
|---|---|---|---|---|---|---|---|---|---|
| Proteinase K proteolysis and LC MS/MS | |||||||||
| RFETGGRISTS | Δm390 | Ser181 | 3+ | 534.2614 | 534.2653 | –7.3 | n/a | ✓ | |
| KVVISTSVGTGL | Δm389 | Ser207 | 3+ | 517.2893 | 517.2943 | –9.7 | n/a | ✓ | |
| KVVISTSVGT | Δm390 | Ser207 | 3+ | 460.9164 | 460.9205 | –8.9 | n/a | ✓ | |
| KVVISTSVGT | Δm389 | Ser207 | 3+ | 460.5884 | 460.5925 | –8.9 | n/a | ✓ | |
| SLVKNDGKDILISGS | Δm389 | Ser343a | 3+ | 645.6748 | 645.6809 | –9.4 | n/a | ✓ | |
| SLVKNDGKDILISGS | Δm390 | Ser343a | 3+ | 646.0026 | 646.0089 | –9.6 | n/a | ✓ | |
| DGKDILISGS | Δm389 | Ser343a | 2+ | 697.3502 | 697.3565 | –9.0 | n/a | ✓ | ✓ |
| LVKNDGKDILISGS | Δm389 | Ser343a | 3+ | 616.6645 | 616.6702 | –9.2 | n/a | ✓ | |
| DGKDILISGS | Δm390 | Ser343a | 2+ | 697.8419 | 697.8485 | –9.4 | n/a | ✓ | ✓ |
| DGKDILISGS | Δm315 | Ser343a | 3+ | 440.5617 | 440.5612 | 1.2 | n/a | ✓ | |
| NDGKDILISGS | Δm389 | Ser343a | 3+ | 503.2496 | 503.2544 | –9.5 | n/a | ✓ | ✓ |
| NDGKDILISGS | Δm315 | Ser343a | 3+ | 478.5761 | 478.5755 | 1.3 | n/a | ✓ | |
| GGYSSVSAY | Δm315, Δm390 | Ser395 Ser398a | 3+ | 532.5648 | 532.5702 | –9.8 | n/a | ✓ | ✓ |
| GGYSSVSAY | Δm315, Δm389 | Ser395 Ser398a | 3+ | 532.2426 | 532.2422 | 0.8 | n/a | ✓ | |
| GSGFSSGSGYSVG | Δm316, Δm315, Δm389 | Ser409, Ser410, Ser412 Ser415 | 3+ | 853.3694 | 853.3766 | –8.4 | n/a | ✓ | |
| NYSTGFAN | Δm389 | Ser423a | 2+ | 631.7742 | 631.7804 | –9.7 | n/a | ✓ | |
| KTTAFGVKDETAGVT | Δm390 | Thr465a | 3+ | 638.9835 | 638.9896 | –9.5 | n/a | ✓ | |
a
Indicates novel glycan and/or glycosylation site. Note that where peptides were identified from both replicates, m/z meas values are given for replicate#2. Glycosylated residues are underlined and in bold.