TABLE 1.
Comparison of capsular genotyping, serotyping by agglutination, and detection of Hib by real-time PCR
| Organism | Strain | Origin of isolatea | Capsular genotypingb | Capsular serotypingb
|
Real-time PCR for Hib | |
|---|---|---|---|---|---|---|
| Difco antisera | Phadebact antisera | |||||
| Reference H. influenzae strainsc (n = 10) | 734 | b | b | b | Positive | |
| 737 | b | b | b | Positive | ||
| 760 | b | b | b | Positive | ||
| 777 | b | b | b | Positive | ||
| 784 | b | b | b | Positive | ||
| 774 | nt | b | nt | Negative | ||
| 776 | nt | b | nt | Negative | ||
| 783 | nt | b | nt | Negative | ||
| 778 | nt | nt | nt | Negative | ||
| 788 | nt | nt | nt | Negative | ||
| Control H. influenzae strains | ATCC 9006 | a | Non-b | Non-b | Negative | |
| cd | Non-b | Non-b | Negative | |||
| ATCC 9332 | d | Non-b | Non-b | Negative | ||
| ATCC 8142 | e | Non-b | Non-b | Negative | ||
| ATCC 9833 | f | Non-b | Non-b | Negative | ||
| Invasive H. influenzae isolates (n = 15) | 158 | BC | b | b | b | Positive |
| 163 | JA | b | b | b | Positive | |
| 339 | CSF | b | b | b | Positive | |
| 426 | JA | b | b | b | Positive | |
| 505 | BC | b | b | b | Positive | |
| 559 | BC | b | b | b | Positive | |
| 572 | BC | b | b | b | Positive | |
| 639 | BC | b | b | b | Positive | |
| 671 | JA | b | b | b | Positive | |
| 990 | BC | b | b | b | Positive | |
| 229 | BC | nt | b | nt | Negative | |
| 509 | CSF | nt | b | nt | Negative | |
| 644 | BC | nt | b | nt | Negative | |
| 380 | CSF | nt | nt | nt | Negative | |
| 386 | BC | nt | nt | nt | Negative | |
a
BC, blood culture; JA, joint aspirate; CSF, cerebrospinal fluid.
b
nt, nontypeable.
c
Reference H. influenzae strains from reference 23.
d
A control H. influenzae type c strain was kindly provided by Loek van Alphen, Amsterdam, The Netherlands.