Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 12;65(8):1377–1388. doi: 10.1136/gutjnl-2014-308506

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

PMC Copyright notice

Inositol-requiring enzyme 1 (IRE1)-dependent induction of pleckstrin homology-like domain, family A, member-3 (PHLDA3). (A) The effect of IRE1 knockdown on PHLDA3 induction. (B) The effect of c-Jun N-terminal kinase (JNK) inhibition on PHLDA3 mRNA, p-c-Jun or p-signal transducer and activator of transcription 3 (STAT3) levels. AML12 cells were exposed to 20 μM JNKi for 1 h and were continuously treated with tunicamycin (Tm) or thapsigargin (Tg). (C) The effects of protein kinase RNA-like endoplasmic reticulum kinase (PERK) or activating transcription factor 6 (ATF6) inhibition. AML12 (left) and HepG2 (right) cells were treated with Tm after transfection with Mock or dominant negative (DN)-PERK construct or with control or ATF6 siRNA. Immunoblottings for p-PERK and ATF6 (90 kDa form) confirmed the effect of DN-PERK and ATF6 siRNA transfection, respectively. For A–C, data represent the mean±SE of three separate experiments. Statistical significance of differences between each treatment group and the control (*p<0.05, **p<0.01) or Tm (^#p<0.05) was determined.