TABLE 2.
List of probe sequences used for Trichosporon species-specific and group-specific identification
| Probe | rDNA region | Specificity | Probe Sequence (5′-3′) | Concn (nM) |
|---|---|---|---|---|
| P1 | D1/D2 | T. brassicae | ATAGCCTAGTATCACATACAC | 0.1 |
| P2 | D1/D2 | T. montevideense/T. domesticum | ATAGCCTAGGTTCACATACAC | 0.1 |
| P3c | IGS | T. scarabeorum | ATTGGCCATATTCCTACTTGC | 0.4 |
| P4 | D1/D2 | T. monoliiforme | TATTATTGCATGCACTGGGTG | 0.1 |
| P5 | D1/D2 | T. jirovecil/T. cutaneum | CAGTCGTGTTCTTCAGATTCA | 0.1 |
| P6 | ITS | T. laibachii/T. multisporum | TGGCTCCTCTCAAAAGAGTTA | 0.1 |
| P7b | IGS | T. multisporum | AGTTCGTACAAGTTCGTGGAT | 0.2 |
| P8 | D1/D2 | T. gracile | GGATAAAGATGCTAGGAATGT | 0.1 |
| P9 | D1/D2 | T. veenhuisii | TTGTCCGGTAGATAAAGGCAG | 0.1 |
| P10 | D1/D2 | T. loubieri | TCAGTTTTGCCCGGTGGATAA | 0.2 |
| P11 | ITS | T. mucoides | ACTTCGGTCGATTACTTTTAC | 0.2 |
| P11b | IGS | T. mucoides | AACGTGCTGCGCTACTAGGTG | 0.4 |
| P12 | ITS | T. cutaneum | TCGCTGGTGTTGACTTGAGAA | 0.1 |
| P12b | IGS | T. cutaneum | CAGTGACATGTGGGCGTTATA | 0.2 |
| P13 | ITS | T. ovoides | GTTTCACTGGTTCCATTGTGT | 0.1 |
| P14 | ITS | T. inkin | CTGGGTCCATGGTGTTGAAGC | 0.1 |
| P15 | D1/D2 | T. asteroides/T. japonicum/T. asahii | TACTTCCTTGGAACGGGTCAA | 0.1 |
| P16b | IGS | T. japonicum | GAGCAGCGAGCGACTTGGCAG | 0.2 |
| P17 | D1/D2 | T. faecale | ACTGCAGCTCACCTTTATGGC | 0.2 |
| P18b | IGS | T. aquatile | CTTAACACGATAACCGGTGCT | 0.2 |
| P19 | D1/D2 | T. caseorum | TTATAGCCTGTTATCACATAC | 0.4 |
| P20 | D1/D2 | T. guehoe | AGGTAGTTTCAATGTAGCTTC | 0.2 |
| P21b | D1/D2 | T. porosum | CATGAATCATGTTTATTGGACTC | 0.1 |
| P22 | ITS | Ovoides cluster | CTGATCGCTCGCCTTAAAAGA | 0.1 |
| P23b | ITS | T. smithiae | TGGATTTGAGTGATGGCAGTT | 0.2 |
| P24 | ITS | T. debeurmannianum | TTTTTACTTCGATCTCAAATC | 0.1 |
| P25b | ITS | T. sporotrichoides | CACTCTGTGTCGATTTTACAA | 0.2 |
| P25c | IGS | T. sporotrichoides | AGTTCATGCTCTAAGTCGGTTC | 0.2 |
| P26 | D1/D2 | T. dehoogii | CCTATTGTCGTATACACTGGA | 0.1 |
| P27b | ITS | Cutaneum cluster | CGGACAATTCTTGAACTCTGGT | 0.2 |
| P28b | IGS | T. jirovecii | TGTGAGTCTATCGGGCGCTTG | 0.2 |
| P29 | ITS | T. lignicola | TGTCCTCTGGAGTAATAAGTT | 0.1 |
| P30 | ITS | Porosum cluster | TGAACGTCTAGTTATTATAACAA | 0.2 |
| P31 | D1/D2 | T. gamsii | ATAAAGGTAATAGGAAGGTGG | 0.2 |
| P32 | D1/D2 | T. laibachii/T. multisporum/T. dulcitum/T. gracile | CAGTCGTGTTTATTGGATTCA | 0.2 |
| P33 | ITS | Gracile cluster | TGAACGTCATTAGATCATAA | 0.1 |
| P34b | IGS | Cryptococcus curvatus | GGTTTAAGATTGTATTGACTG | 0.2 |
| P35 | IGS | T. dulcitum | AACGTACAAGTCCGGACATGA | 0.2 |
| P36 | IGS | T. dermatis | ACGGTAGTTTGGAGTGAAGTGC | 0.2 |
| P37 | IGS | T. asteroides/T. japonicum | GAGCAGCGAGCGACTTGGCAG | 0.2 |
| P38 | IGS | T. asahii | TTCTCGACTGATGGCCTTGGT | 0.2 |
| P39 | IGS | T. montevideense | GGGTCCTAATAGATGCCATGT | 0.2 |
| P41b | IGS | T. coremiiforme | CACAGAGGTGCAAGAGGTGGG | 0.2 |
| P42 | IGS | T. lactis | GCTTCGGAGACTTGGGTTTGC | 0.4 |
| P43 | ITS | T. inkin/T. ovoides | ACTGTTCTACCACTTGACGCA | 0.4 |
| P44b | D1/D2 | T. vadense | TTATCACATGCACTGGGGGAG | 0.4 |
| P46 | IGS | T. domesticum | GCATGAGTGACGGCAGAGGTG | 0.4 |
| P47 | IGS | T. laibachii | GTGCACAAGAAACATACTAAC | 0.2 |