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. 2016 Aug 5;15:136. doi: 10.1186/s12934-016-0534-3

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Refolding and purification of insoluble peptides. The refolding and purification results of different peptides, designated with Leptin, CCL5, SDF1, and IGF1. Lane IC, insoluble fraction after intein-mediated cleavage; lane SR, soluble fraction after refolding of the insoluble peptides; lane PU, final product of target peptide after RP-HPLC purification; lanes 1, 2, and 3, quantification standards (Std) consisting of bovine serum albumin (BSA, 66.5 kDa) at 3, 1.5, and 0.75 µg/lane and aprotinin (6.5 kDa) at 1.5, 0.75, and 0.3 µg/lane, respectively. The molecular masses of the protein standards M1 and M2 are listed by the left and right side separately