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. Author manuscript; available in PMC: 2016 Aug 6.
Published in final edited form as: Head Neck. 2015 Jun 16;38(2):237–246. doi: 10.1002/hed.23877

Figure 2.

Figure 2

Silk-elastin-like protein polymer (SELP) preserves GLV-1h68 viral integrity at body temperature. (A) Viral elution from the SELP gel for plaque assays was optimized by the use of a shaking incubator to disrupt the gel. Shaking enhanced viral recovery from SELP gel, as compared with vortexing, or shaking with homogenizer beads. (B) GLV-1h68 viral integrity was measured in SELP versus phosphate-buffered saline (PBS), when maintained in an incubator at 37°C over a 4-week period in the absence of any cells. Viral integrity was measured by measuring infectious viral titers. (C) Green fluorescent protein (GFP) expression by plaque assay samples demonstrates enhanced late viral integrity and transgene expression by the GLV-1h68 in SELP as compared with PBS. The red circles in the SELP group denote tiny gel particles created by the shaking incubator. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]