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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: J Immunol. 2016 Jul 8;197(4):1425–1434. doi: 10.4049/jimmunol.1600264

Figure 5. Depletion of monocytes limits disease severity in G2A−/− mice and reduces eosinophil numbers.

Figure 5

Mice were treated with anti-CCR2 clone MC-21 (MC-21) or isotype antibody as described in Experimental Procedures. A, monocytes were depleted with MC-21: Left, representative flow plot demonstrating efficient depletion of monocytes. Right, total monocytes were significantly reduced in MC-21 treated animals (versus isotype treated) for both genotypes. B, Disease activity index (DAI) was determined in MC-21 or isotype treated mice over the timecourse of DSS exposure (left) and in an expanded representation for day 6 of DSS exposure (right). Dotted line indicates maximum DAI score. C, colon length was determined at day 6 following administration of DSS in MC-21 and isotype treated animals. Dotted line represents colon length of water control animals. D, total numbers of eosinophils were determined with and without monocyte depletion with MC-21. Data represent mean ± SEM n=10, *p<0.05 and **p<0.01 compared to WT isotype, WT MC-21 and G2A−/− MC-21.