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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: J Immunol. 2016 Jul 15;197(4):1322–1334. doi: 10.4049/jimmunol.1600406

Figure 4. GABAA and NMDA receptors do not mediate the inhibitory effects of ethanol.

Figure 4

IL-1β and TNF ELISAs from the supernatants of J774 cells treated with LPS for 8 h and picrotoxin, ethanol and ATP for 1 h (A), LPS for 8 hours followed by ethanol and ATP for 1 hour, with the addition of muscimol at the time of LPS or ethanol and ATP treatment (B), or LPS for 8 h and CPP, ethanol and ATP for 1 hour (C). *<0.0001 by a Tukey’s post hoc test following a one-way ANOVA relative to the LPS+ethanol (3 or 1.2%)+ATP treated group (A) or the LPS+ATP treated group (B and C). EtOH = ethanol, CPP = 3-(2-Carboxypiperazin-4-yl)propyl-1-phosphonic acid, ns = not significant. All studies were performed in triplicate.