TABLE 2.
Reproducibility and accuracy of the TaqMan HCV ASR determined with a standard reference panel of HCV RNA
Labeled concn (IU/ml) | Measured concna (IU/ml) | SD | CV (%) | Bias (log10) |
---|---|---|---|---|
2,000,000 | 6,950,000 | 862,300 | 12 | 0.54 |
500,000 | 1,050,000 | 159,400 | 15 | 0.32 |
200,000 | 538,000 | 70,220 | 13 | 0.43 |
50,000 | 109,000 | 3610 | 3 | 0.34 |
5,000 | 12,200 | 1940 | 16 | 0.39 |
500 | 1,500 | 390 | 26 | 0.48 |
50 | 6 | 9 | 53 | −0.92 |
Each concentration was tested in quadruplicate. HCV RNA was detected in all of the replicates.