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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: J Immunol. 2016 Jul 6;197(4):1353–1367. doi: 10.4049/jimmunol.1600699

FIGURE 7. Pannexin-1, P2X7R, and certain TRP channel family members are not required for caspase-1-dependent pyroptotic pore induction.

FIGURE 7

(A) LPS-primed WT BMDM were stimulated with NG (10μM) for 45 min in the presence or absence of the pannexin 1 inhibitor trovafloxacin (30μM). These data represent the mean ± SE of 4 replicates from 2 independent experiments. (B) LPS-primed WT BMDM were stimulated with NG for 45 min in the presence of the P2X7R antagonists A10606120 (10μM) or A438079 (10μM). These data represent the mean ± SE of 4 replicates from 2 independent experiments. (C) LPS-primed WT or P2X7R−/− BMDM were stimulated with NG or (D) TcdB (0.4μg/mL) for 45 min. These data represent the mean ± SE of 3 replicates from 1 experiment. (A–D) Propidium2+ fluorescence was quantified every 3 (NG) or 4 (TcdB) min as described in Fig. 1. (E) LPS-primed WT or P2X7R−/− BMDM were stimulated with NG. At the indicated times, supernatants were assayed for LDH activity as described in Fig. 1. These data represent the mean ± SE of 2 replicates from 1 experiment. (F) LPS-primed WT BMDM were stimulated with NG for 45 min or (G) TcdB for 60 min in the presence of the TRPV channel inhibitor ruthenium red (RR: 1μM) or the TRPM7 channel inhibitor NS8593 (30μM). These data represent the mean ± SE of 2–4 replicates from 2 (F) or 1 (G) independent experiments. (F–G) Propidium2+ fluorescence was quantified every 3 (NG) or 4 (TcdB) min as described in Fig. 1.