Table 3.
Primer sequences and accession numbers
| Primer | Accession Numbers | Oligo | Sequence |
|---|---|---|---|
| β-Actin | NM_031144.3 | Forward | 5’-GTCGTACCACTGGCATTGTG-3’ |
| Reverse | 5’-GCCATCTCTTGCTCGAAGTC-3’ | ||
| C-Fos | NM_022197.2 | Forward | 5’-CCAAGCGGAGACAGATCAAC-3’ |
| Reverse | 5’-AAGTCCAGGGAGGTCACAGA-3’ | ||
| CyclophilinA | NM_017101.1 | Forward | 5’-GCGTCTGCTTCGAGCTGTTT-3’ |
| Reverse | 5’-CGTAGATGGACTTGCCACCA-3’ | ||
| Gapdh1 | NM_017008 | Forward | 5’-ATGACTCTACCCACGGCAAG-3’ |
| Reverse | 5’-AGCATCACCCCATTTGATGT-3’ | ||
| Hmgb-12 | NM_012963.2 | Forward | 5’-GGCGGCTGTTTTGTTGACAT-3’ |
| Reverse | 5’-ACCCAAAATGGGCAAAAGCA-3’ | ||
| Hsp723 | NM_031971.2 | Forward | 5’-GGCCTTGAGGACTTTGGGTT-3’ |
| Reverse | 5’-CTGGGAATGCAAAGCACACG-3’ | ||
| IκBα4 | NM_001105720.2 | Forward | 5’-CTGTTGAAGTGTGGGGCTGA-3’ |
| Reverse | 5’-AGGGCAACTCATCTTCCGTG-3’ | ||
| Il-1β5 | NM_031512 | Forward | 5’-AGGACCCAAGCACCTTCTTT-3’ |
| Reverse | 5’-AGACAGCACGAGGCATTTTT-3’ | ||
| Il-66 | NM_012589 | Forward | 5’-TAGTCCTTCCTACCCCAACTTCC-3’ |
| Reverse | 5’-TTGGTCCTTAGCCACTCCTTC-3’ | ||
| Mcp-17 | NM_031530.1 | Forward | 5’-TCTCTGTCACGCTTCTGGG-3’ |
| Reverse | 5’-TGCTGCTGGTGATTCTCTTG -3’ | ||
| Tnf-α8 | NM_012675 | Forward | 5’-GGGGCCACCACGCTCTTCTG-3’ |
| Reverse | 5’-CGACGTGGGCTACGGGTTG-3’ |
Primers, accession numbers, and sequences used in real-time RT-PCR for all gene expression studies.
1
Glyceraldehyde 3-phosphate dehydrogenase;
2
High-mobility group box 1;
3
Heat shock protein 72;
4
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha;
5
Interleukin-1 beta;
6
Interleukin-6;
7
Monocyte chemotactic protein 1;
8
Tumor necrosis factor alpha.