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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: J Immunol. 2016 Jul 15;197(4):1343–1352. doi: 10.4049/jimmunol.1502357

Table 1.

PCR primers used in the generation of TLR9471-1032-HA and TLR9441-1032-HA

Construct Forward Reverse
mTLR9471-1032-HA Primer1
5′-GGCCCGCCTGGCATTATGCCCAG-3′
Primer 2
5′-CAGGTCCATGGTGAACTTGAAGTTCTTACAGTCACCAGTGGAACCTGGAACCCAGAGCAGCAG-3′
Primer 3
5′-CTGCTGCTCTGGGTTCCAGGTTCCACTGGTGACTGTAAGAACTTCAAGTTCACCATGGAGCTG-3′
Primer 4
5′-TATCTCGAGCTAAGCGTAGTCTGGGACGTCGTATGGG-3′

mTLR9441-1032-HA Primer 1
5′-GGCCCGCCTGGCATTATGCCCAG-3′
Primer 2
5′-CAACAGCTCCTCCTGCTCTGCATCATCTGCGTCACCAGTGGAACCTGGAACCCAGAGCAGCAG-3′
Primer 3
5′-CTGCTGCTCTGGGTTCCAGGTTCCACTGGTGACGCAGATGATGCAGAGCAGGAGGAGCTGTTG-3′
Primer 4
5′-TATCTCGAGCTAAGCGTAGTCTGGGACGTCGTATGGG-3′