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(A) RT-PCR of IRF2BP2 showed increased transcripts in the proband versus healthy control (versus actin) with agreement between primers specific for exon 1 (present in isoform 1 of IRF2BP2) and exon 2 (present in both isoforms 1 and 2). (B) Western blot showed increased protein expression of IRF2BP2 in lysates of EBV-LCL from the proband versus control. TATA Binding protein is shown as loading control.
