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PCR analysis using the MSP3-MSP4B primer pair specific to the SSU rRNA gene of E. bieneusi. Lane M, molecular markers of 100-bp DNA ladder; lane 1, negative control; lanes 2 to 6, different concentrations of spores (100,000, 20,000, 4,000, 800, and 160 spores/ml, respectively); lane 7, positive control.
