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. 2016 Aug 8;7:1150. doi: 10.3389/fpls.2016.01150

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Copyright © 2016 Conde, Pimentel, Neves, Dinis, Bernardo, Correia, Gerós and Moutinho-Pereira.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of kaolin application in the transcript levels of grapevine stilbene synthase 1 (VvSTS1) in mature and full mature grape berries. Gene expression analysis was performed by real-time qPCR in grape berry tissues collected from vines subjected to kaolin treatment and without application (control). VvSTS1 relative expression levels were obtained after normalization with the expression of the reference genes VvACT1 and VvGAPDH. Two independent PCR runs with triplicates were performed for each tested mRNA. Values are the mean ± SEM. Asterisks indicate statistical significance (Student's t-test; ^*P < 0.05.