TABLE 2.

Oligonucleotide used in this study

Adapter, primer or probe	Sequence	Amplicon size (bp)
Adapters
RBam12	5′-GATCCTCGGTGA-3′
RBam24	5′-AGCACTCTCCAGCCTCTCACCGAG-3′
JBam12	5′-GATCCGTTCATG-3′
JBam24	5′-ACCGACGTCGACTATCCATGAACG-3′
Primers
Sgo215F	5′-GGTGTTGTTTGACCCGTTCAG-3′	96
Sgo310R	5′-AGTCCATCCCACGAGCACAG-3′
Smi168F	5′-GAGTCCTGCATCAGCCAAGAG-3′	96
Smi263R	5′-GGATCCACCTTTTCTGCTTGAC-3′
Ana209F	5′-TCGAAACTCAGCAAGTAGCCG-3′	96
Ana304R	5′-AGAGGAGGGCCACAAAAGAAA-3′
Avi251F	5′-ATGTGGGTCTGACCTGCTGC-3′	96
Avi346R	5′-CAAAGTCGATCACGCTCCG-3′
Smu3368F	5′-GCCTACAGCTCAGAGATGCTATTCT-3′	114
Smu3481R	5′-GCCATACACCACTCATGAATTGA-3′
Sso287F	5′-TTCAAAGCCAAGACCAAGCTAGT-3′	88
Sso374R	5′-CCAGCCTGAGATTCAGCTTGT-3′
Probes
Sgo237T	5′-FAM-AACCTTGACCCGCTCATTACCAGCTAGTATG-TAMRA-3′a
Smi201T	5′-FAM-TGTTCCCAAGTGGAGCCAACCAAACT-TAMRA-3′
Ana249T	5′-FAM-GGGTACTCTAGTCCAAACTGGCGGATAGCG-TAMRA-3′
Avi272T	5′-FAM-ACGGAGGTCGGGAACGGTGGAAG-TAMRA-3′
Smu3423T	5′-FAM-TGGAAATGACGGTCGCCGTTATGAA-TAMRA-3′
Sso298T	5′-FAM-CCTGCTCCAGCGACAAAGGCAGC-TAMRA-3′

^a6-FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.