TABLE 3.
Sequences obtained by subtractive hybridization
| Species | Fragment size (bp) | Region containing ORFa | Predicted protein (aa)b | % GC content | Best match in database (homologue) | E valuec |
|---|---|---|---|---|---|---|
| S. gordonii | 437 | 3-407 | 134 | 46.4 | Putative type I site-specific DNase (Streptococcus pyogenes M1) | 7e − 37 |
| S. mitis | 401 | 173-400 | 75 | 44.2 | Glycine-rich protein (Arabidopsis thaliana) | 0.83 |
| A. naeslundii | 355 | 2-337 | 111 | 43.1 | Unknown protein (Mesorhizobium loti) | 1.6 |
| A. viscosus | 440 | 120-439 | 99 | 64.2 | Unknown protein (Oryza sativa) | 0.25 |
a
The regions corresponding to the predicted open reading frames (ORFs) were searched using the ORF finder (http://www.ncbi.nlm.nih.gov/gorf/gorf.html).
b
The number of amino acids in the predicted protein is shown.
c
Highest scoring match in GenBank with the blastx algorithm.