Table 1.
Primer sets used for PCR detection of HCN and control mRNAs
| Gene | GenBank Accession | Forward (5′ → 3′) | Reverse (5′ → 3′) | Amplicon Size, bp | Exon Location |
|---|---|---|---|---|---|
| RT-PCR Primers | |||||
| HCN1 | NM_053375.1 | TTGGAGAAATATGCCTGCTG | GTCCACCGAAAGGGAGTAAA | 89 | 6–7 |
| HCN2 | NM_053684.1 | ACAACATGGTGAACCACTCG | CCAATACAGAGCATGTGGCT | 79 | 3–4 |
| HCN3 | NM_053685.1 | CGCTTCGGGAGGAGATTAT | GTACTGCGGTGACGAAACTG | 96 | 5–6 |
| HCN4 | NM_021658.1 | ATCAACGGCATGGTGAATAA | CGTCCGTACCCAATACACAG | 92 | 3–4 |
| NPY | NM_012614.2 | TGGCCAGATACTACTCCGCT | ATGGAAGGGTCTTCAAGCCT | 154 | 2–3 |
| qPCR Primers | |||||
| HCN1 | CTTCGGATCGTGAGGTTTAC | TGCACTGGCGAGATCATA | 123 | 2–3 | |
| HCN2 | CGTGCGTTTCACCAAGA | ATGCTGATCAGGTTACAGATG | 144 | 2–3 | |
| HCN3 | CCTGCGCATCGTTAGATT | GGTCGTAGGTCATGTGAAAG | 113 | 2–3 | |
| HCN4 | AGCAGGAGAGGGTTAAGTCA | CATCAGCAACAGCATCGTCA | 92 | 1–2 | |
| GAPDH | NM_017008.4 | TCTCTGCTCCTCCCTGTTC | GTAACCAGGCGTCCGATAC | 120 | 1–3 |