TABLE 1.

Characteristics of primers used for suicide PCR in our study

Yr and primer name	Gene name (gene product)	Primer sequence (5′-3′)	Amplicon size (bp)a	Hybridization temp (°C)
1998
lpxD1Fb	lpxD [UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase]	ATATAGGAATAGTTAAAATCGGC	315	56
lpxD1Rc		TAGATTGTCTATGCCAACCAT
lpxD2Fbd		AACACTACTATTGATAGAGG	106	50
lpxD2Rcd		GCTACTTCCTGCTATACC
1999
fabZ1Fb	fabZ [3R-hydroxymyristoyl-(acyl carrier protein)]	TTAGTAGATAGAGTACTTAAAATC	264	56
fabZ1Rc		CTTGAAAAYTTCCATACGTTAGC
fabZ2Fbd		AAATCGATCCTAACAAATCAATAA	225	50
fabZ2Rcd		TCTTTGCTGATCAATAACAG
1999
yidC1Fb	yidC (60-kDa inner membrane protein)	TAGAATCTGAATCACTTACCGG	401	57
yidC1Rc		CCGATAGGTCCTTGATGTAATA
yidC2Fbd		CATTAAAAGGACTTAGATTTG	153	50
yid2Rcd		TTTCACTATCACTATTCCATA
2000
pcnB1Fb	pcnB [poly(A) polymerase]	CTAATTCTAATTTAAAATYATTTGC	401	56
pcnB1Rc		AAAGATATTGAATGTAACGGYAG
pcnB2Fbd		CATAATTTTGKATAGAGAATAT	342	50
pcnB2Rcd		TTTTGCCGAAGATGCCG
2001
recF1Fb	recF (RecF protein)	GAACTAAAAACAGATAATACACC	316	56
recF1Rc		TCTATCAAGAAATTTTCTTCTATC
recF2Fbd		GGTAGCGGTAAAACTAATAT	245	50
recF2Rcd		GCTTGTAAAGATTCCTTCCA
2002
gltX1Fb	gltX (Glutamyl-tRNA synthetase)	AGTTATAACACGMTTTGCTCC	533	56
gltX1Rc		ATCAATATCATCAATAACTGAGC
gltX2Fbd		ACATTTAATCAATTAAGTCGT	285	50
gltX2Rcd		TCTGCTCTTATCACTATTGG
2003
mutL1Fb	mutL (DNA mismatch repair protein MutL)	GTAAAGGAATTAGTTGAAAATGCT	420	55
mutL1Rc		GGATGTGCTAGAGCGATTTT
mutL2Fbd		AACGTCATACAACTTCTAAG	166	50
mutL2Rcd		AGTACCTTCATTATGAACAG

^aThe sizes of the amplicons are given with reference to the “R. conorii conorii” strain Malish (Seven) genome sequence (GenBank accession number NC_003103).

^bForward primer.

^cReverse primer.

^dPrimer used for reamplification.