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. 2004 Aug;42(8):3799–3804. doi: 10.1128/JCM.42.8.3799-3804.2004

TABLE 4.

E. coli isolates used for evaluation of E. coli O114-specific PCR

Culture no. Strain O group H type Origin and yr of isolationa Sourceb Virulence markersc
G1331 C 319-58 O114 H10 UK, 1957 HF None
G1358 C 556-59 O114 H10 Sweden, 1959 Calf septicemia None
G1376 C 288-63 O114 H2 Germany, 1959 HF, D LA, eaf, bfp, eae
G1378 C 276-63 O114 H2 Germany, 1960 HF, D LA, eaf, bfp, eae
G1366 C 280-63 O114 H2 Germany, 1961 HF, D LA, eaf, bfp, eae
G1367 C 281-63 O114 H2 Germany, 1961 HF, D LA, eaf, bfp, eae
G1365 C 290-63 O114 H2 Germany, 1959 HF, D LA, eaf, bfp, eae
G1339 C 315-60 O114 H2 UK, 1960 HF, D LA, eaf, bfp, eae
G1377 C 289-63 O114 H2 Germany, 1958 HF, D LA, eaf, bfp, eae
G1379 C 275-53 O114 H21 Egypt, 1953 HF ST
G1361 C 339-54 O114 H21 Israel, 1954 HF LT
G1336 C 1003-63 O114 H21 Iran, 1963 HF ST
G1359 1098/63 O114 H32 Germany, 1963 Pig feces None
G1360 C 240-58 O114 H32 Denmark, 1958 Pig feces None
G1356 26 W O114 H32 Sweden, 1944 calf septicemia None
G1351 CB 9421 O114 H34 Brazil, 2002 HF, D eae
G1373 C 2870/67 O114 H4 Germany, 1967 Monkey None
G1374 C 3142/71 O114 H4 Germany, 1971 HF None
G1375 C 3143/71 O114 H4 Germany, 1971 HF None
G1341 3075/69 O114 H4 Germany, 1969 HF, D Stx1
G1349 C 637-62 O114 H4 Italy, 1962 Calf septicemia None
G1340 707/59 O114 H4 UK, 1959 Pig feces None
G1343 CB 177 O114 H4 New Zealand, 1986 HF None
G1313 C 2924-68 O114 H4 Germany, 1968 HF None
G1362 C 340-53 O114 H49 Israel, 1953 HF LT
G1325 IP 831 O114 H49 Tunesia, 1984 HF LT
G1371 C 3813 O114 H9 Germany, 1978 HF F12-fim, alpha-hly
G1372 C 4011 O114 H9 Germany, 1983 HF F12-fim
G1350 C 4155 O114 H9 Germany, 1984 HF F12-fim, alpha-hly
G1353 C 311-58 O114 H9 Sweden, 1958 HF F12-fim
G1330 539/83 O114 H9 Germany, 1980 HF, D F12-fim, alpha-hly
G1337 CB 176 O114 H9 New Zealand, 1986 HF F12-fim
G1305 C 2837-67 O114 H9 Germany, 1967 HF F12-fim
G1342 C 4462 O114 ND Germany, 1989 HF None
G1338 C 4519 O114 ND Germany, 1990 HF eae
G1355 C 4557 O114 ND Germany, 1990 HF None
G1333 3140 O114 NM Germany, 1971 HF None
G1364 4154 O114 NM Germany, 1984 HF, D F12-fim, alpha-hly
G1347 3008/69 O114 NM Germany, 1969 HF, D Alpha-hly
G1321 CB 9605 O114 NM Germany, 2003 HF, D eae
G1308 C 1962 O114 NM Germany, 1962 HF None
G1345 CB 9723 O145 H28 Germany, 2003 HF, D EHEC-hly, eae
G1328 CB 9805 O145 NM Germany, 2003 HF, D eae
G1334 CB 9759 O157 H7 Germany, 2003 HF, D EHEC-hly, Stx1+2, eae
G1329 CB 9777 O157 H7 Germany, 2003 HF, D EHEC-hly, Stx1+2, eae
G1363 CB 9468 O157 NM Germany, 2003 HF, D EHEC-hly, Stx1+2, eae
G1324 CB 4364 O172 ND Germany, 1995 HF ND
G1323 CB 7132 O172 ND Switzerland, 1997 Unknown ND
G1322 CB 7133 O172 ND Switzerland, 1998 Unknown ND
G1309 2533-54 O175 ND ND ND ND
G1326 CB 9776 O180 H2 Germany, 2003 HF, D eae
G1311 E39a O23 H15 — Reference strain ND
G1344 CB 9720 O26 H11 Germany, 2003 HF, D EHEC-hly, eae
G1354 CB 9748 O26 H11 Germany, 2003 HF, D EHEC-hly, eae
G1352 CB 9752 O26 H11 Germany, 2003 HF, D EHEC-hly, eae
G1332 CB 9772 O28 NM Germany, 2003 HF, D eae
G1303 U8-41 O48d NM — Reference strain ND
G1302 U1-41 O5d H4 — Reference strain ND
G1315 P9c O70d H42 — Reference strain ND
G1319 P10a O71d H12 — Reference strain ND
G1304 H14 O82d NM — Reference strain ND
G1314 C 600 O-rough H48 E. coli K-12 Laboratory strain None
G1335 CB 9767 O100 H25 Germany, 2003 HF, D eae
G1327 CB 9801 O103 H2 Germany, 2003 HF, D eae
G1306 Bi 623-42 O11 H10 — Reference strain ND
G1318 CB 6911 C. freundiid ND Germany, 1997 HF, D None
a

—, E. coli serotype reference strain (19).

b

HF, human feces; D, diarrhea; ND, no data.

c

The virulence markers LT (heat-labile enterotoxin), ST (heat-stable enterotoxin), presence of the EAF plasmid, localized adherence to HEp-2 cells (LA), alpha-hemolysin, and presence of P (F12-related) fimbriae of E. coli O114 strains were tested by DNA hybridization (LT, ST, and EAF), colony immunoblot (alpha-hemolysin), and latex agglutination tests (P-fimbriae) as described previously (7, 8). The presence of the Shiga toxins, enterohemorrhagic E. coli (EHEC) hemolysin, the intimin gene (eae), and the bundle-forming pili (bfp) gene was investigated by PCR as described previously (6, 24). None, indicates that none of the virulence factors investigated in this study were detected.

d

O serogroups reported to show serological cross-reactivity with the O114 antigen (12, 16).