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Instrument settings for CTM48 ASR assaya
| Step | Ramp slope | Temperature (°C) | Cycle time (s) | Cycle delay (s) |
|---|---|---|---|---|
| Cover heating | 3 | 100.0 | — | — |
| Precycle | 12 | 50.0 | 300 | 0 |
| Precycle | 12 | 59.0 | 1,800 | 0 |
| Sequence start | — | — | — | — |
| Denaturation | 12 | 95.0 | 15 | 0 |
| Annealing | 12 | 58.0 | 25 | 25 |
| Sequence end | 2 | — | — | — |
| Sequence start | — | — | — | — |
| Denaturation | 12 | 91.0 | 15 | 0 |
| Annealing | 12 | 58.0 | 25 | 25 |
| Sequence end | 60 | — | — | — |
| Postcycle | 12 | 40.0 | 120 | 0 |
—, program step with no required parameter.
